Composite

Part:BBa_K2906016:Design

Designed by: Sara ElSafadi, Ewan Egan   Group: iGEM19_Manchester   (2019-10-15)


T7 promoter- RBS- COMT- T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 141
    Illegal AgeI site found at 324
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the gene is plant-derived, had to codon optimise it using GeneArt. His-Tag fused after cloning into pETDuet-1 plasmid.



Source

Sequence comes from Arabidopsis thaliana (UniProtKb P21964).


References